Work package 5

WP5 will employ in vitro, ex vivo and in vivo methodologies to elucidate the clinical potential of the advanced wound care materials in regards to safety, diagnostics, and wound healing. The safety and efficacy of treatment using the developed wound dressing materials will be evaluated using state-of-the art model systems, and the effect of the novel dressing material on three aspects will be evaluated; 1) proliferation of human keratinocytes and fibroblasts, 2) migration of human keratinocytes and fibroblasts, and 3) healing process of human tissue ex vivo wounds. Further evaluation of toxicology/safety will be performed using a porcine wound healing model. We have extensive experience of the suggested model system in the context of wound healing, dressing materials, infection, and transplantation, and a thorough understanding of its potential and limitations. The work in work package 5 will prepare for the clinical validation of the novel wound dressings developed in the HEALiX project.

References:

• Junker, J.P., et al., Topical delivery of ultrahigh concentrations of gentamicin is highly effective in reducing bacterial levels in infected porcine full-thickness wounds. Plast Reconstr Surg, 2015. 135(1): p. 151-9
• Tsai, D.M., et al., Full-thickness porcine burns infected with Staphylococcus aureus or Pseudomonas aeruginosa can be effectively treated with topical antibiotics. Wound Repair Regen, 2016. 24(2): p. 356-65.
• Hackl, F., et al., Moist dressing coverage supports proliferation and migration of transplanted skin micrografts in full-thickness porcine wounds. Burns, 2014. 40(2): p. 274-80